Antiproliferiative Activity of Biogenic Silver Nanoparticles Synthesized from Leonotis nepetifolia (L) on Human Cancer Cell lines

Currently, nanobiotechnology is the growing science in field of medicine employing silver nanoparticles to deliver drugs target cancer cells and proved be potential anticancer agents. In this study, were synthesized using leaf extract Leonotis nepetifolia (L). Gas chromatography-mass spectrometry data showed a total 13 phytochemical constituents identified from ethanolic The effect physicochemical factors such as reaction time, temperature, concentration Silver nitrate, pH synthesis nanoparticles. For biosynthesis, optimal conditions 1 mM 0.1 ml at 80° for 90 min. Ultra violet-visible spectroscopy, emission scanning electron microscopy; high-resolution transmission microscopy various techniques characterized AgNPs. obtained had absorbance maxima 398 nm, spherical shape, an average particle size 11.32 polydispersity index 0.44, zeta -23.75.69 mv crystalline. A cytotoxicity study was conducted on two human cell lines, pancreatic ovarian, sulforhodamine B assay. We compared biogenic with that synthetic Treatment ovarian different concentrations inhibited viability half maximal inhibitory values 25.4 μg/ml 23.9 μg/ml. contrast, 48.2 μg/ 42 μg/ml, which statistically significant (p<0.001), when doxorubicin Camptothecin. mediated effectively reduced proliferation lines than